BBCSport Lukas Rosol could not maintain the stellar form that overcame Rafael Nadal and went down in straight sets to Germany’s Philipp Kohlschreiber.
Rosol pulled off one of the biggest upsets in history when he knocked out the two-time champion on Centre Court, but lost 6-2 6-3 7-6 to Kohlschreiber.
The 26-year-old returned to form more akin to his ranking of 100, losing the first set in 25 minutes on Court 12.
Rosol saved one match point in the tie-break but lost in 96 minutes.
Meanwhile, Defending champion Petra Kvitova and four-time champion Serena Williams progresses to the fourth round.
Williams survived a scare to complete a 6-7 (5-7) 6-2 9-7 win over Jie Zheng while Kvitova eased through with a 6-1 6-0 victory over Varvara Lepchenko.
